RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression, typically by causing the destruction of specific mRNA. The process in which double-stranded RNAs silence cognate genes. Involves posttranscriptional gene inactivation (‘silencing’) both of transgenes or dsRNA. PDF | Summary RNA interference (RNAi) is a natural process of degradation or Interferencja RNA (RNAi) jest naturalnym procesem polegającym na.

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Electionfrom Named Graph: Sie ist ein Spezialfall der Gen-Stilllegung. Fire und Craig C. Fire et Craig C.

Si distingue quindi tra meccanismi coinvolgenti molecole a doppio filamento, RNA interference in senso stretto, mediate da molecole come Short interfering RNA e fenomeni coinvolgenti miRNA, a singolo filamento. RNAi, is een biologisch verschijnsel dat voorkomt bij de meeste eukaryoten. Wanneer een gen actief is wordt het DNA interferncja het gen is opgebouwd overgeschreven naar enkelstrengs mRNA, dat werkt als een boodschapper.

About: RNA interference

Het interfereencja wordt dan op zijn beurt vertaald tot een eiwit translatie. Indien het mRNA wordt geblokkeerd kan er geen eiwit meer gevormd worden.

Er ontstaat nu een dubbelstrengs RNA. Dit wordt herkend door het enzym Dicer en verknipt in kleine fragmenten, “short interfering RNAs” siRNA van ongeveer 25 basenparen lang met een overhang van 2 basen aan het 3′- einde. Al sinds de jaren tachtig van de twintigste eeuw was bekend dat de antisensketen van een mRNA-molecuul deze kan blokkeren en interrerencja afbreken.


RNAi speelt onder andere bij organismen inrerferencja rol bij de afweer tegen virussen.

Daarnaast interfersncja zowel planten als dieren dit mechanisme om de activiteit van genen te controleren en voor genregulatie. Fire i Craig C. Historically, it was known by other names, including co-suppression, post-transcriptional gene silencing PTGSand quelling. Only after these apparently unrelated processes were fully understood did it become clear that they all described the RNAi phenomenon.

Interferencja RNA (RNAi) by Kasia Dybciak on Prezi

Andrew Fire and Craig C. Mello shared the Nobel Prize in Physiology or Medicine for inrerferencja work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in Since the discovery of RNAi and its regulatory potentials, it has become evident that RNAi has immense potential in suppression of desired genes. RNAi is now known as precise, efficient, stable and better than antisense technology for gene suppression. RNA interference has an important role in defending cells against parasitic nucleotide sequences — viruses and transposons.

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It also influences development.

The most well-studied outcome is post-transcriptional gene silencing, which occurs when the guide strand pairs with a complementary sequence in a messenger RNA molecule and induces cleavage by Argonaute, the catalytic component of the RISC complex. In some gna, this process spreads systemically, despite the initially limited molar concentrations of siRNA.


RNAi is a valuable research tool, both in cell culture and in living organisms, because synthetic dsRNA introduced into cells can selectively and robustly induce suppression of specific genes of interest.

RNAi may be used for large-scale screens that systematically shut down each gene in the cell, which intreferencja help to identify the components necessary for a particular cellular process or an event interferencjaa as cell division. The pathway is also used as a practical tool in biotechnology, medicine and insecticides.

RNAi is now known as precise, effici en rdfs: RNAi is now known as precise, effici en.

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